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ABL(Y253H)

Anti-ABL(Y253H) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26154

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   ABL(Y253H)
货号:   26154
规格:   100 µL
基因符号:   ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl
描述:   ABL(Y253H) 小鼠单抗
背景:   V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia.
免疫原 :   A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin.
经过测试的应用:   ELISA, WB, IHC, IF
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:  Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   recognizes Abl Y253H, but not wild type of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:   

Western blot analysis of recombinant Abl(Y253H) and wild type proteins. Purified His-tagged Abl(Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-ABL(Y253H) 小鼠单抗 (货号:26154).

Immunofluorescence:   
Immunofluorescence of cells expressing Abl proteins with anti-Abl(Y253H) antibody.

HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl(Y253H) plasmid (right column), then fixed and stained with anti-ABL(Y253H) monoclonal antibody (货号:26154).

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