武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Vimentin mAb

Anti-Vimentin Mouse Monoclonal Antibody
5/5

$2,800.00

货号:26054

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   Vimentin 小鼠单抗
货号:   26054
基因符号:   VIM
描述:   Vimentin 小鼠单抗
背景:   Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
免疫原 :   A synthetic peptide from the internal region of human vimentin protein.
经过测试的应用:   ELISA, WB, IHC, IP
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:  Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   vimentin antibody recognizes vimentin of vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:   

Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (货号:10190) was blotted with Anti-vimentin monoclonal antibody (货号:   26054).
lmmunohistochemistry:

Immunohistochemical analysis of paraffin-embedded human renal cancer tissue with anti-vimentin monoclonal antibody (货号:26054). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100 dilution) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
           引用文献