武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

GRB7 mAb

anti-GRB7 Mouse Monoclonal Antibody
5/5

$2,800.00

货号:26133

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   GRB7 小鼠单抗
货号:   26133
基因符号:   GRB7
描述:   GRB7 小鼠单抗
背景:   Grb7 is an adaptor protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. It plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Grb7 gene is commonly co-amplified (present in excess copies) in breast cancers. Grb7 is over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers. There are clues that it involved in cell migration and metastatic progression of human esophageal carcinomas.
免疫原 :   Purified GRB7 protein, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
Concentration:    0.5 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:  Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes GRB7 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:   

Western blot analysis of recombinant GRB7 protein. Purified His-tagged GRB7 protein was blotted with anti GRB7 小鼠单抗 (货号:26133).
Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti GRB7 monoclonal antibody (货号:26133). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
           引用文献