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SARS-CoV-2 (Beta) S protein RBD, hFc Tag

Recombinant SARS-CoV-2 S protein RBD(K417N, E484K,N501Y) protein with C-terminal human Fc tag
5/5

$445.00

货号:11339

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11339
产品全名:   SARS-CoV-2 (Beta) S Protein RBD
规格:   10/50/100 µg
基因符号   SARS-CoV-2 B.1.351 (Beta) Spike RBD 蛋白
目标蛋白:    S protein RBD
UNIPROT ID:    P0DTC2
描述:   “Recombinant SARS-CoV-2 S protein RBD(K417N
背景:     SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of RBD(K417N, E484K,N501Y)-hFc is approximately 55-70 kDa due to glycosylation.
分子特征:   S protein RBD(K417N, E484K,N501Y)(Arg319-Phe541) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD (K417N, E484K,N501Y), hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Beta) S protein RBD, hFc Tag (11339) can bind Human ACE2 Protein, mFc Tag (11195) in a linear range of 12.346-3000 ng/mL.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Beta) S protein RBD, hFc Tag (11339) can bind Anti-SARS-CoV-2 RBD antibody (DM55), Rabbit mAb (28354) in a linear range of 0.244-15.625 ng/mL.
           引用文献