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Rab35(Q67L) Protein

Rab35 Protein Q67L mutant
5/5

$248.00

货号:10133

规格: 25 微升

库存状态:In Stock

           产品描述          
产品名称:   Rab35(Q67L) 突变蛋白
货号:   10133
产品全名:   Rab35 Protein Q67L 突变蛋白
基因符号  Member RAS oncogene family, RAY, H-ray, RAB1C
Source:   Human, recombinant full length, His6-tag
Expression 种属反应性:   E. coli
分子量:   23 kDa
纯化:   >95% by SDS-PAGE
Introduction:   The Ras super-family of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, the Rab family appears to play a critical role in regulating exocytotic and endocytotic pathways. RAB35 regulates the assembly of actin filaments during bristle development in Drosophila and filopodia formation in cultured cells.
Amino Acid Sequence  (1-201, Q67L)
MARDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGL
ERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVVETED
AYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):   White or clear
Concentration: 1 mg/mL
Storage:    -80°C
Preparation Instructions:   
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab35 QL was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.


References:   
1. Zhang, J. et al., Science 325: 1250-1254, 2009.
2. Zhu, A. X. et al., Biochem. Biophys. Res. Commun. 205: 1875-1882, 1994.

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