武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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RAS(A146T)

Anti-RAS(A146T) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26261

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   RAS(A146T) 小鼠单抗
货号:   26261
基因符号:   H-Ras; K-Ras; N-Ras
描述:   RAS(A146T) 小鼠单抗
背景:   The A146T mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146T mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
免疫原:   A synthetic peptide from the internal region of Ras which includes the mutation of A146T, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
Preservative:  No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes A146T mutant, but not wild type RAS of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant HRAS(A146T) and wild type proteins. Purified His-tagged RAS(A146T) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-RAS(A146T) monoclonal antibody (货号:26261).Immunofluorescence:  Immunofluorescence of cells expressing HRas proteins with anti-HRAS(A146T) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRAS(A146T) plasmid (right column), then fixed and stained with anti-HRAS(A146T) monoclonal antibody (货号:26261).