武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

13-GTP

Anti-Gα13-GTP Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26902

规格: 30 微升

库存状态:In Stock

           产品描述          
产品名称:   13-GTP 小鼠单抗
货号:   货号:: 26902
规格:   30 µL
基因符号:   Gna13
描述:   13-GTP 小鼠单抗
背景:   Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
免疫原:   Recombinant full length active Gα13 蛋白
经过测试的应用:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
推荐稀释比:   IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG2b
纯化:   Purified from ascites
Preservative:  No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:  IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP 小鼠单抗 (货号:   26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 小鼠单抗 (货号:   26004).
Immunohistochemistry:  Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.