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PIK3CA(D549N)

Anti-PIK3CA(D549N) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26215

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   PIK3CA(D549N) 小鼠单抗
货号:   26215
规格:   100 µL
基因符号:   PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
描述:   PIK3CA(D549N) 小鼠单抗
背景:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
免疫原:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   0.5 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes D549N mutant, but not wild type PIK3CA of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant PIK3CA(D549N) and wild type proteins. Purified His-tagged PIK3CA(D549N) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(D549N) monoclonal antibody (货号:26215).
Immunofluorescence:  Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(D549N) plasmid (right column), then fixed and stained with Anti-PIK3CA(D549N) monoclonal antibody (货号:26215).
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