武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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KIT(W557R)

Anti-KIT(W557R) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26275

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   KIT(W557R) 小鼠单抗
货号:   26275
基因符号:   C-Kit; CD117; KIT; PBT; SCFR
描述:   KIT(W557R) 小鼠单抗
背景:   Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.
免疫原:   A synthetic peptide from the internal region of KIT which includes the mutation of W557R, human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IF: 1:50-1:100IHC: 1:25-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes W557R mutant, but not wild type KIT of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant KIT(W557R) and wild type proteins.Purified His-tagged KIT(W557R) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-KIT(W557R) monoclonal antibody (货号:26275).Immunofluorescence:  Immunofluorescence of cells expressing KIT proteins with Anti-KIT(W557R) antibody.HEK293T cells were transfected with pCDNA3-GFP-KIT (WT) plasmid (left column) or pCDNA3-GFP-KIT(W557R) plasmid (right column), then fixed and stained with Anti-KIT(W557R) monoclonal antibody (货号:26275).
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