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Human TNFa Protein, His Tag

Recombinant human TNFa protein with C-terminal 6XHis tag
5/5

$525.00

货号:11199

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11199
产品全名:   人 TNFa 蛋白
规格:   10/50/100 µg
基因符号   DIF;NF-alpha;NFA;NFSF2;achexin;achectin;NFa
目标蛋白:    TNFA
UNIPROT ID:    P01375
描述:   Recombinant human TNFa protein with C-terminal 6xHis tag
背景:     This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.
分子特征:   TNFa(Val77-Leu233) 6×His tag
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human TNFα Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human TNFa , His tagged protein (11199) can bind Anti-TNFa Neutralizing antibody 28064 in a linear range of 0.64-16 ng/ml.
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