武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human CD33(140-259) Protein, hFc Tag

Recombinant human CD33 protein with C-terminal human Fc tag
5/5

$389.00

货号:11489

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11489
产品全名:   人 CD33(140-259) 蛋白
规格:   10/50/100 µg
基因符号   CD33;IGLEC3;p67
目标蛋白:    CD33
UNIPROT ID:    P20138
描述:   Recombinant human CD33 protein with C-terminal human Fc tag
背景:     Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 38.9 kDa after removal of the signal peptide.
分子特征:   CD33(Asp140-His259) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD33, hFc Tag on SDS-PAGE under reducing condition.
           引用文献