| 货号: 11374 |
| 产品全名: 人 CD112 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 NECTIN2;HVEB;PRR2;PVRL2;PVRR2 |
| 目标蛋白: CD112 |
| UNIPROT ID: Q92692 |
| 描述: Recombinant Human CD112 with C-terminal mouse Fc tag |
| 背景: This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 61.4 kDa after removal of the signal peptide.The apparent molecular mass of CD112-mFc is approximately 35-55 kDa due to glycosylation. |
| 分子特征: CD112(Gln32-Gly360) mFc(Pro99-Lys330) |
| 纯化: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD112 Protein, mFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. Flow cytometry analysis with 15µg/ml Human CD112 Protein, mFc tag (11374) on Expi293 cells transfected with human PVRIG (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). | 
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD112 Protein, mFc Tag(11374) can bind Anti-CD112 antibody(DMC224), IgG1 Chimeric mAb in a linear range of 3.20-80 ng/mL. | |
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