武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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EGFR(L858R)

Anti-EGFR(L858R) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26082

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   EGFR(L858R) 小鼠单抗
货号:   26082
基因符号:   EGFR/HER1/ERBB1
描述:   EGFR(L858R) 小鼠单抗
背景:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
免疫原:   A synthetic peptide from the internal region of EGFR which includes the mutation of L858R, human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:1000-1:5000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG1
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes EGFR(L858R), but not wild type EGFR from vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing.
蛋白质印迹:  Immunohistochemical analysis of paraffin-embedded human NSCLC tissue with anti-EGFR(L858R) monoclonal antibody (货号:26082).Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:400) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:100) was used as secondary antibody.