背景:    EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT,PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).

Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858Q, human origin.

Tested applications: ELISA, WB, IF, IHC

推荐稀释比:

ELISA:   1:1000-1:5000

WB:    1:500-1:1000

IHC:    1:50-1:100

Concentration: 1 mg/ml

种属反应性: Mouse

克隆性： Monoclonal

纯化: Purified from ascites

形式: Liquid

Storage buffer: 

Preservative: no

成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性: Recognizes EGFR (L858Q),but not wild type EGFR protein from vertebrates.

储存条件:    Store at -20°C. Avoid repeated freezing and thawing.