武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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CTCF(K344E)

Anti-CTCF(K344E) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26324

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   CTCF(K344E) 小鼠单抗
货号:   26324
基因符号:   CTCF; MRD21
描述:   CTCF(K344E) 小鼠单抗
背景:   Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture.
免疫原 :   A synthetic peptide from the internal region of CTCF which includes the mutation of K344E, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:  Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes K344E mutant, but not wild type CTCF of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:   

Western blot analysis of recombinant CTCF (K344E) and wild type proteins.

Purified His-tagged CTCF (K344E) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-CTCF(K344E) monoclonal antibody (货号:26324).

Immunofluorescence:   

Immunofluorescence of cells expressing CTCF proteins with Anti-CTCF(K344E) antibody.

HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (K344E) plasmid (right column), then fixed and stained with Anti-CTCF(K344E) monoclonal antibody (货号:26324).

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