武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human CCR2 Full Length Protein

Human CCR2 Full Length Protein-Synthetic Nanodisc
5/5

$30,000.00

货号:11011

规格: 50 μg

库存状态:In Stock

           产品描述          
货号:   11011
产品全名:   人 CCR2 Full Length 蛋白
规格:   10/50/100 µg
基因符号   CC-CKR-2; CCR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R
目标蛋白:    CCR2
UNIPROT ID:    P41597
描述:   人 CCR2 full length protein-synthetic nanodisc
背景:     The protein is a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The encoded protein mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This protein can also be a coreceptor with CD4 for HIV-1 infection. This gene is located in the chemokine receptor gene cluster region of chromosome 3.
物种/宿主:    HEK293
分子量:   The human full length CCR2 protein has a MW of 41.9 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CCR2-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CCR2-Nanodisc is 7.62ng/ml.

Figure2. Human CCR2-Nanodisc, Flag Tag on SDS-PAGE

Figure3. WB analysis of Human CCR2-Nanodisc with anti-CCR2 monoclonal antibody (28090), followed by Goat Anti-Human IgG HRP at 1/5000 dilution
           引用文献