武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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AKT1(E49K)

Anti-AKT1(E49K) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26137

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   AKT1(E49K) 小鼠单抗
货号:   26137
规格:   100 µl
基因符号:   AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
描述:   AKT1(E49K) 小鼠单抗
背景:   AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.
免疫原:   A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:2000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes E49K mutant, but not wild type AKT1 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant AKT1(E49K) and wild type proteins.Purified His-tagged AKT1(E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-AKT1(E49K) monoclonal antibody (货号:26137).
Immunofluorescence:   Immunofluorescence of cells expressing AKT1 proteins with Anti-AKT1(E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP-AKT1 (WT) plasmid (left column) or pCDNA3-GFP-AKT1(E49K) plasmid (right column), then fixed and stained with Anti-AKT1(E49K) monoclonal antibody (货号:26137).
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