武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human TLR4 Full Length Protein

Human TLR4 Full Length Protein-Synthetic Nanodisc
5/5

$30,000.00

货号:11122

规格: 50 μg

库存状态:In Stock

           产品描述          
货号:   11122
产品全名:   人 TLR4 Full Length 蛋白
规格:   10/50/100 µg
基因符号   ARMD10; CD284; TLR-4
目标蛋白:    TLR4
UNIPROT ID:    O00206
描述:   人 TLR4 full length protein-synthetic nanodisc
背景:     The protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration.
物种/宿主:    HEK293
分子量:   The human full length TLR4 protein has a MW of 95.7 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag TLR4-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TLR4-Nanodisc is 2.939ng/ml.

Figure2. Human TLR4-Nanodisc, Flag Tag on SDS-PAGE
           引用文献