武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

SMAD4 mAb

Anti-SMAD4 Mouse Monoclonal Antibody
5/5

$2,800.00

货号:26453

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   SMAD4 小鼠单抗
货号:   26453
基因符号:   SMAD4, DPC4, JIP, MADH4, MYHRS
描述:   SMAD4 小鼠单抗
背景:   SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-β  signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominant disease juvenile polyposis syndrome (JPS).
免疫原:   Purified SMAD4 protein, human origin. .
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:2000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes SMAD4 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant SMAD4 protein. Purified His-tagged SMAD4 protein (1-552aa, 65kd) and was blotted with anti-SMAD4 monoclonal antibody (货号:26453).Immunofluorescence:  Immunofluorescence of cells expressing SMAD4 proteins with anti SMAD4 antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4 (WT) plasmid, then fixed and stained with anti SMAD4 monoclonal antibody (货号:26453).
           引用文献