武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

SARS-CoV-2 (Delta) S protein RBD , hFc Tag

Recombinant SARS-CoV-2 RBD(L452R,T478K) protein with C-terminal human Fc tag
5/5

$445.00

货号:11454

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11454
产品全名:   SARS-CoV-2 (Delta) S Protein RBD
规格:   10/50/100 µg
基因符号   SARS-CoV-2 B.1.617.2 (Delta) Spike RBD 蛋白
目标蛋白:    S protein RBD
UNIPROT ID:    P0DTC2
描述:   “Recombinant SARS-CoV-2 RBD(L452R
背景:     SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 51.2 kDa after removal of the signal peptide.The apparent molecular mass of RBD(L452Rand T487K)-hFc is approximately 55-70 kDa due to glycosylation.
分子特征:   S protein RBD(L452Rand T487K)(Arg319-Phe541) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD(L452R & T487K), hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Delta) S protein RBD , hFc Tag (11454) can bind Human ACE2 Protein, mFc Tag 11195 in a linear range of 4.115-3000 ng/mL.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Delta) S protein RBD , hFc Tag (11454) can bind Anti-SARS-CoV-2 (CB6 biosimilar) mAb 28020 in a linear range of 1.00-97.66 ng/mL.

Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Delta) S protein RBD , hFc Tag (11454) can bind Anti-SARS-CoV-2 RBD antibody (DM55), Rabbit mAb 28354 in a linear range of 0.244-15.625 ng/mL.
           引用文献