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SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag

Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal 6XHis tag
5/5

$525.00

货号:11164

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11164
产品全名:   SARS-CoV-2 (2019-NCoV) S Protein RBD
规格:   10/50/100 µg
基因符号   S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD;COVID-19
目标蛋白:    S protein RBD
UNIPROT ID:    P0DTC2
描述:   Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal 6xHis tag
背景:     SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 37-38 kDa after removal of the signal peptide.
分子特征:   S protein RBD(Arg319-Phe541) 6×His tag
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, His tagged protein (11164) can bind its native ligand Human ACE2, hFc tagged protein 11196 in a linear range of 1.96-31.72 ng/ml.
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