武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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RAS(G13V)

Anti-RAS(G13V) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26477

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   RAS(G13V) 
货号:   26477
基因符号:   H-Ras; K-Ras; N-Ras
描述:   RAS(G13V) 小鼠单抗
背景:   Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
免疫原:   A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes G13V mutant, but not wild type RAS of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant Ras proteins. Purified His-tagged RAS(G13V) protein (lane 1), corresponding wild type protein (lane 2), His-tagged Ras (G13S) protein (lane 3), His-tagged Ras (G13C) protein (lane 4), His-tagged Ras (G13R) protein (lane 5), His-tagged Ras (G13A) protein (lane 6) were blotted with Anti-RAS(G13V) monoclonal antibody (货号:26477).Immunofluorescence:  Immunofluorescence of cells expressing Ras proteins with Anti-RAS(G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13V) plasmid,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid, pCDNA3-GFP-Ras (G13C) plasmid, pCDNA3-GFP-Ras (G13R) plasmid, pCDNA3-GFP-Ras (G13A) plasmid or pCDNA3-GFP-Ras (G13D) plasmid, then fixed and stained with Anti-RAS(G13V) monoclonal antibody (货号:26477).