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Human NPC1L1 Full Length Protein

Human NPC1L1 Full Length Protein-Synthetic Nanodisc
5/5

¥30,000.00

货号:11070

规格: 50 μg

库存状态:3-4 weeks

           产品描述          
货号:   11070
产品全名:   人 NPC1L1 Full Length 蛋白
规格:   10/50/100 µg
基因符号   LDLCQ7; NPC11L1; SLC65A2
目标蛋白:    NPC1L1
UNIPROT ID:    Q9UHC9
描述:   人 NPC1L1 full length protein-synthetic nanodisc
背景:     The protein is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk.
物种/宿主:    HEK293
分子量:   The human full length NPC1L1 protein has a MW of 148.7 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag NPC1L1-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with NPC1L1-Nanodisc is 4.526ng/ml.

Figure2. Human NPC1L1-Nanodisc, Flag Tag on SDS-PAGE
           引用文献