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MEK1(N382H)

Anti-MEK1(N382H) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26270

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   MEK1(N382H) 
货号:   26270
基因符号:   MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1
描述:   MEK1(N382H) 小鼠单抗
背景:   MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.
免疫原:   A synthetic peptide from the internal region of MEK1 which includes the mutation of N382H, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes N382H mutant, but not wild type MEK1 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant MEK1(N382H) and wild type proteins. Purified His-tagged MEK1(N382H) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-MEK1(N382H) monoclonal antibody (货号:26270).Immunofluorescence:  Immunofluorescence of cells expressing MEK1 proteins with Anti-MEK1(N382H) antibody. HEK293T cells were transfected with pCDNA3-GFP-MEK1 (WT) plasmid (left column) or pCDNA3-GFP-MEK1(N382H) plasmid (right column), then fixed and stained with Anti-MEK1(N382H) monoclonal antibody (货号:26270).
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