武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: IDH2(R140Q) 小鼠单抗 |
货号: 26165 |
基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
描述: IDH2(R140Q) 小鼠单抗 |
背景: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
免疫原 : A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin. |
经过测试的应用: ELISA, WB, IF, IHC |
推荐稀释比: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: |
纯化: Purified from ascites |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes R140Q mutant, but not wild type IDH2 of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant IDH2(R140Q) and wild type proteins.
Purified His-tagged IDH2(R140Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with anti IDH2(R140Q) monoclonal antibody (货号:26165).
Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R140Q) antibody.
HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R140Q) plasmid (right column), then fixed and stained with anti-IDH2(R140Q) monoclonal antibody (货号:26165).