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Human TIM3 Protein, mFc-His Tag

Recombinant human TIM3 protein with C-terminal mouse Fc and 6XHis tag
5/5

$525.00

货号:11158

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11158
产品全名:   人 TIM3 蛋白
规格:   10/50/100 µg
基因符号   HAVCR2;TIM3;TIMD3;FLJ14428;KIM3
目标蛋白:    TIM3
UNIPROT ID:    Q8TDQ0
描述:   Recombinant human TIM3 protein with C-terminal mouse Fc and 6xHis tag
背景:     The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 47.5 kDa after removal of the signal peptide.
分子特征:   TIM3(Ser22-Gly202) mFc(Pro99-Lys330) 6×His tag
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human TIM3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human TIM3, mFc-His tagged protein (11158) can bind Anti-TIM3 Neutralizing antibody 28017 in a linear range of 0.64-80 ng/ml.
           引用文献