武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human TIGIT Protein, hFc Tag

Recombinant human TIGIT Protein with C-terminal human Fc tag
5/5

$525.00

货号:11225

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11225
产品全名:   人 TIGIT 蛋白
规格:   10/50/100 µg
基因符号   TIGIT;SIG9;STM3
目标蛋白:    TIGIT
UNIPROT ID:    Q495A1
描述:   Recombinant human TIGIT Protein with C-terminal human Fc tag
背景:     This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity, this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 39.13 kDa after removal of the signal peptide.The apparent molecular mass of TIGIT-hFc is approximately 40-53 kDa due to glycosylation.
分子特征:   TIGIT(Met22-Pro141) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human TIGIT Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD155, mFc-His tagged protein 11155 can bind Human TIGIT, hFc tagged protein (11225) in a linear range of 1.95-125 ng/ml.

Figure 3.  Flow cytometry analysis with 1µg/ml Human TIGIT Protein, hFc tag (11225) on Expi293 cells transfected with human CD112 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
           引用文献