| 货号: 12057 |
| 产品全名: 人 IL-2 Superkine (突变蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 Interleukin-2;IL-2;T-Cell Growth Factor;TCGF;Aldesleukin;IL2 |
| 目标蛋白: IL-2 |
| UNIPROT ID: P60568 |
| 描述: “Recombinant Human Interleukin-2 Superkine is produced by our Mammalian expression system and the target gene encoding Ala21-Thr153 (L100F |
| 背景: Interleukin-2(IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system,belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. New research has shown that IL-2 mutant reduced toxicity while being more potent at stimulating anti-tumor effector immune cells. |
| 物种/宿主: HEK293 |
| 分子量: 16.5 KDa |
| 分子特征: Not available |
| 纯化: Greater than 95% as determined by reducing SDS-PAGE. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Greater than 95% as determined by reducing SDS-PAGE. | 
Figure 2. Loaded Human IL-2RB-Fc on Protein A Biosensor, can bind Human IL-2 Superkine with an affinity constant of 0.58 nM as determined in BLI assay. | 
Figure 3. Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is 151.39 pg/ml. | 
Figure 4. Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Human IL-2 Superkine with an affinity constant of 10.4 nM as determined in BLI assay. |
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