| 货号: 12089 |
| 产品全名: 人 IL-15RAandIL-15 Complex (C-Fc) 蛋白 |
| 规格: 10 µg; 50 µg and 100 µg |
| 基因符号 IL-15 Receptor alpha and IL-15 Fusion Protein;IL15RAandIL15;IL15RAandIL15 Complex;Interleukin-15;IL-15;IL15 |
| 目标蛋白: IL-15RAandamp;IL-15 |
| UNIPROT ID: P29459;P29460 |
| 描述: Recombinant Human Interleukin-15 Receptor Alpha And Interleukin-15 Fusion Protein Complex Is Produced By Our Mammalian Expression System And The Target Gene Encoding Ile31-Asp96AndAsn49-Ser162 (Asn120Asp) Is Expressed With A Fc Tag At The C-Terminus. |
| 背景: IL15RA is a high-affinity receptor for interleukin-15. Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide variety of Tand B cells and non-lymphoid cells.IL-15 is a cytokine that regulates T cell and natural killer cell activation and proliferation. IL-15 binds to the alpha subunit of the IL-15RA with high affinity. IL-15 also binds to the beta and gamma chains of the IL-2 receptor, but not the alpha subunit of the IL2 receptor. IL-15 is structurally and functionally related to IL-2. Both cytokines share some subunits of receptors, allowing them to compete for and negatively regulate each other's activity. The number of CD8 memory T cells is controlled by a balance between IL-15 and IL-2. Despite their many overlapping functional properties, IL-2 and IL-15 are, in fact, quite distinct players in the immune system. IL-15 is constitutively expressed by a wide variety of cell types and tissues, including monocytes, macrophages and DCs. The enhanced activity of the IL-15N72D:IL-15RaSu/Fc complex is likely the result of the increased binding activity of IL-15N72D to IL-15Rβγ c , optimized cytokine trans-presentation by the IL-15RaSu domain, the dimeric nature of the cytokine domain and its increased in vivo half-life compared to IL-15. These findings indicate that this IL-15 superagonist complex could serve as a superior immunostimulatory therapeutic agent. |
| 物种/宿主: HEK293 |
| 分子量: 34.4and12.8 KDa |
| 分子特征: Not available |
| 纯化: Greater than 95% as determined by reducing SDS-PAGE. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HC, 150 mM NaC, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Greater than 95% as determined by reducing SDS-PAGE. |
