武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human HVEM Protein, His Tag

Recombinant Human HVEM Protein with C-terminal 6XHis tag
5/5

$555.00

货号:11253

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11253
产品全名:   人 HVEM 蛋白
规格:   10/50/100 µg
基因符号   ATAR;CD270;HVEA;HVEM;LIGHTR;TR2
目标蛋白:    HVEM
UNIPROT ID:    Q92956
描述:   Recombinant Human HVEM Protein with C-terminal 6xHis tag
背景:     This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide.The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation.
分子特征:   HVEM(Leu39-Val202) 6×His tag
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human HVEM Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human BTLA,mFc-His tagged protein 11174 in a linear range of 0.64-80 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human CD160,hFc tagged protein 11179 in a linear range of 1.28-160 ng/ml.

Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human LIGHT, hFc tagged protein 11175 in a linear range of 0.32-40 ng/ml.

Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
           引用文献