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Human EGFR Protein, His Tag

Recombinant human EGFR Protein with C-terminal 6XHis tag
5/5

$479.00

货号:11218

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11218
产品全名:   人 EGFR 蛋白
规格:   10/50/100 µg
基因符号   EGFR;RBB;RBB1;ER1;IG61;ENA
目标蛋白:    EGFR
UNIPROT ID:    P00533
描述:   Recombinant human EGFR Protein with C-terminal 6xHis tag
背景:     The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 69.4 kDa after removal of the signal peptide.The apparent molecular mass of EGFR-His is approximately 100-130 kDa due to glycosylation.
分子特征:   EGFR(Leu25-Ser645) 6×His tag
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human EGFR Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human EGFR, His tagged protein (11218) can bind Anti-EGFR Antibody 28043 in a linear range of 0.64-80.0 ng/ml.
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