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Human CD28 Protein, mFc-His Tag

Recombinant human CD28 protein with C-terminal mouse Fc and 6XHis tag
5/5

$555.00

货号:11142

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11142
产品全名:   人 CD28 蛋白
规格:   10/50/100 µg
基因符号   CD28;Tp44
目标蛋白:    CD28
UNIPROT ID:    P10747
描述:   Recombinant human CD28 protein with C-terminal mouse Fc and 6xHis tag
背景:     The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide.
分子特征:   CD28(Asn19-Pro152) mFc(Pro99-Lys330) 6×His
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein 11283 can bind Human CD28, mFc-His tagged protein (11142) in a linear range of 125-4000 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-2, hFc tagged protein 11284 can bind Human CD28, mFc-His tagged protein (11142)  in a linear range of 62.5-4000 ng/ml.

Figure 4. HEK293 cell line transfected with irrelevant protein  (B) and human B7-2  (A) were surface stained with Human CD28, mFc-His tagged protein (11142) 1µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
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