武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human CB1 (1-116) Protein, hFc Tag

Recombinant Human CB1 protein with C-terminal human Fc
5/5

$415.00

货号:11310

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11310
产品全名:   人 CB1 (1-116) 蛋白
规格:   10/50/100 µg
基因符号   CANN6;CB-R;CB1;CB1A;CB1K5;CB1R;CNR
目标蛋白:    CB1
UNIPROT ID:    P21554
描述:   Recombinant Human CB1 protein with C-terminal human Fc
背景:     This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide.The apparent molecular mass of CB1-hFc is approximately 55-70 kDa due to glycosylation.
分子特征:   CB1(Met1-Gln116) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CB1 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CB1(1-116) Protein, hFc Tag (11310) can bind Anti-CB1 antibody(DM144), Rabbit mAb in a linear range of 3.20-16 ng/mL.
           引用文献