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Human B7-H4 Protein, hFc Tag

Recombinant Human B7-H4 protein with C-terminal human Fc
5/5

$445.00

货号:11177

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11177
产品全名:   人 B7-H4 蛋白
规格:   10/50/100 µg
基因符号   B7-H4;B7h.5;B7H4;B7S1;B7X;PRO1291;VCTN1
目标蛋白:    B7-H4
UNIPROT ID:    Q7Z7D3
描述:   Recombinant Human B7-H4 protein with C-terminal human Fc
背景:     This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide.The apparent molecular mass of B7-H4-hFc is approximately 100-130 kDa due to glycosylation.
分子特征:   B7-H4 (Phe29-Ala258) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human B7-H4 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human B7-H4, hFc-His tagged protein  (11177) can bind Anti-B7-H4 Neutralizing antibody 28081 in a linear range of 3.2-80 ng/ml.
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