武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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Human AFP Protein, hFc Tag

Recombinant human AFP protein with C-terminal human Fc tag
5/5

$445.00

货号:11198

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   11198
产品全名:   人 AFP 蛋白
规格:   10/50/100 µg
基因符号   AFPD;FETA;HPAFP
目标蛋白:    AFP
UNIPROT ID:    P02771
描述:   Recombinant human AFP protein with C-terminal human Fc tag
背景:     This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
物种/宿主:    HEK293
分子量:   The protein has a predicted molecular mass of 92.6 kDa after removal of the signal peptide.The apparent molecular mass of AFP-hFc is approximately 100-130 kDa due to glycosylation.
分子特征:   AFP(Arg19-Val609) hFc(Glu99-Ala330)
纯化:   The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human AFP Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AFP Protein, hFc Tag(11198) can bind Anti-AFP antibody(DMC281), IgG1 Chimeric mAb in a linear range of 0.64-16 ng/mL.
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