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Human GCGR Full Length Protein

Human GCGR Full Length Protein-Synthetic Nanodisc
5/5

$30,000.00

货号:11066

规格: 50 μg

库存状态:3-4 weeks

           产品描述          
货号:   11066
产品全名:   人 GCGR Full Length 蛋白
规格:   10/50/100 µg
基因符号   GGR; GL-R; MVAH
目标蛋白:    GCGR
UNIPROT ID:    P47871
描述:   人 GCGR full length protein-synthetic nanodisc
背景:     G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
物种/宿主:    HEK293
分子量:   The human full length GCGR protein has a MW of 54.0 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag GCGR-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GCGR-Nanodisc is 3.800ng/ml.

Figure2. Human GCGR-Nanodisc, Flag Tag on SDS-PAGE
           引用文献