武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: FBXW7(R479L) 小鼠单抗 |
货号: 26347 |
基因符号: FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10 |
描述: FBXW7(R479L) 小鼠单抗 |
背景: F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene’s potential role in the pathogenesis of human cancers. |
免疫原: A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin. |
经过测试的应用: ELISA, WB |
推荐稀释比: ELISA: 1:1000-1:5000WB: 1:500-1:1000 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯化: Purified from ascites |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes R479L mutant, but not wild type FBXW7 of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant FBXW7(R479L) and wild type proteins.Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-FBXW7(R479L) monoclonal antibody (货号:26347).Immunofluorescence: Immunofluorescence of cells expressing FBXW7 proteins with Anti-FBXW7(R479L) antibody.HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with Anti-FBXW7(R479L) monoclonal antibody (货号:26347). |