武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human F2RL1 Full Length Protein

Human F2RL1 Full Length Protein-Synthetic Nanodisc
5/5

¥30,000.00

货号:11018

规格: 50 μg

库存状态:In Stock

           产品描述          
货号:   11018
产品全名:   人 F2RL1 Full Length 蛋白
规格:   10/50/100 µg
基因符号   GPR11; PAR2
目标蛋白:    F2RL1
UNIPROT ID:    P55085
描述:   人 F2RL1 full length protein-synthetic nanodisc
背景:     A member of the G-protein coupled receptor 1 family of proteins. The encoded cell surface receptor is activated through proteolytic cleavage of its extracellular amino terminus, resulting in a new amino terminus that acts as a tethered ligand that binds to an extracellular loop domain. Activation of the receptor has been shown to stimulate vascular smooth muscle relaxation, dilate blood vessels, increase blood flow, and lower blood pressure. This protein is also important in the inflammatory response, as well as innate and adaptive immunity.
物种/宿主:    HEK293
分子量:   The human full length F2RL1 protein has a MW of 44.1 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag F2RL1-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with F2RL1-Nanodisc is 2.32ng/ml.

Figure2. WB analysis of Human F2RL1-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
           引用文献