武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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ERBB2(V777A)

Anti-ErbB2(V777A) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26080

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   ErbB2(V777A) 小鼠单抗
货号:   26080
基因符号:   ERBB2/HER2/Neu
描述:   ErbB2(V777A) 小鼠单抗
背景:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers.
免疫原:   A synthetic peptide surround the 777A codon of ErbB2 protein.
Tested Applications:   ELISA, WB, IF, IHC
推荐稀释比:   ELISA: 1:500-1:5000WB: 1:200-1:2000IF: 1:100IHC: 1:50-1:100
Concentration:   0.2 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing.
Immunofluorescence:  Immunofluorescence of cells expressing ErbB2 proteins with Anti-ErbB2(V777A) antibody.HEK293T cells were transfected with pCDNA3-GFP- ErbB2 WT plasmid (left column) or pCDNA3-GFP-ErbB2(V777A) plasmid (right column), then fixed and stained with Anti-ErbB2(V777A) monoclonal antibody (货号:26080).
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