武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

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ERBB2 mAb

Anti-ERBB2 Mouse Monoclonal Antibody
5/5

$2,800.00

货号:26127

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   ERBB2 小鼠单抗
货号:   26127
基因符号:   ERBB2/HER2/Neu/c-Neu
描述:   ERBB2 小鼠单抗
背景:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important criteria in clinic evaluating of the progression of breast cancer.
免疫原:   A synthetic peptide from the C-terminal region of ERBB2, human origin
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:2000IHC: 1:50-1:200
Concentration:    1mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes Erb-B2 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant Erb-B2 protein. Purified His-tagged Erb-B2 protein (货号:10208) was blotted with anti-Erb-B2 monoclonal antibody (货号:26127).Immunohistochemistry:  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-Erb-B2 monoclonal antibody (货号:26127). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
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