武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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EGFR(S768I)

Anti-EGFR(S768I) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26483

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   EGFR(S768I) 小鼠单抗
货号:   26483
基因符号:   EGFR/HER1/ERBB1
描述:   EGFR(S768I) 小鼠单抗
背景:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
免疫原 :   A synthetic peptide from the internal region of EGFR which includes the mutation of S768I, human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:1000-1:5000
IHC: 1:50-1:200
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:  Monoclonal
亚型:   
纯化:   Purified from ascites
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes EGFR (S768I), but not wild type EGFR from vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:   

image.png

Western blot analysis of recombinant EGFR (S768I) and wild type proteins.

Purified His-tagged EGFR (S768I) protein (lane 1) and corresponding wild type protein (amino acids 702-987, lane 2) were blotted with anti EGFR(S768I) 小鼠单抗 (货号:26483).
Immunofluorescence:   

image.png

Immunofluorescence of cells expressing EGFR proteins with anti EGFR(S768I) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(S768I) plasmid (right column), then fixed and stained with anti- EGFR (S768I) monoclonal antibody (货号:26483).

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