武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

CTNNB1(D32N)

Anti-CTNNB1(D32N) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26303

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   CTNNB1(D32N) 
货号:   26303
基因符号:   Beta-catenin, CTNNB, Catenin beta-1
描述:   CTNNB1(D32N) 小鼠单抗
背景:   CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherent junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers.
免疫原:   A synthetic peptide from the internal region of CTNNB1 which includes the mutation of D32N, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes D32N mutant, but not wild type CTNNB1 of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant CTNNB1 (D32N) and wild type proteins.Purified His-tagged CTNNB1 (D32N) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-CTNNB1(D32N) monoclonal antibody (货号:26303).Immunofluorescence:  Immunofluorescence of cells expressing CTNNB1 proteins with Anti-CTNNB1(D32N) antibody.HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (D32N) plasmid (right column), then fixed and stained with Anti-CTNNB1(D32N) monoclonal antibody (货号:26303).
           引用文献