武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

Human CD37 Full Length Protein

Human CD37 Full Length Protein-Synthetic Nanodisc
5/5

¥30,000.00

货号:11023

规格: 50 μg

库存状态:In Stock

           产品描述          
货号:   11023
产品全名:   人 CD37 Full Length 蛋白
规格:   10/50/100 µg
基因符号   GP52-40; TSPAN26
目标蛋白:    CD37
UNIPROT ID:    P11049
描述:   人 CD37 full length protein-synthetic nanodisc
背景:     The protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms.
物种/宿主:    HEK293
分子量:   The human full length CD37 protein has a MW of 31.7 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CD37-Nanodisc (0.2 µg/per well). Serial diluted anti-CD37 monoclonal antibody (28055) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD37 monoclonal antibody binding with CD37-Nanodisc is 23.06ng/ml.

Figure2. Human CD37-Nanodisc, Flag Tag on SDS-PAGE

Figure3. WB analysis of Human CD37-Nanodisc with anti-CD37 monoclonal antibody (28055), followed by Goat Anti-Human IgG HRP at 1/5000 dilution
           引用文献