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Human CCR5 Full Length Protein

Human CCR5 Full Length Protein-Synthetic Nanodisc
5/5

$30,000.00

货号:11081

规格: 50 μg

库存状态:In Stock

           产品描述          
货号:   11081
产品全名:   人 CCR5 Full Length 蛋白
规格:   10/50/100 µg
基因符号   CC-CKR-5; CCCKR5; CCR-5; CD195; CKR-5; CKR5; CMKBR5; IDDM22
目标蛋白:    CCR5
UNIPROT ID:    P51681
描述:   人 CCR5 full length protein-synthetic nanodisc
背景:     A member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele.
物种/宿主:    HEK293
分子量:   The human full length CCR5 protein has a MW of 40.5 kDa
Formulation & Reconstitution:   Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CCR5-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CCR5-Nanodisc is 4.18ng/ml.

Figure2. Human CCR5-Nanodisc, Flag Tag on SDS-PAGE
           引用文献