武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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BRAF(G469E)

Anti BRAF(G469E) Mouse Monoclonal Antibody
5/5

$4,800.00

货号:26196

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   BRAF(G469E) 小鼠单抗
货号:   26196
基因符号:   BRAF, BRAF1, RAFB1
描述:   BRAF(G469E) 小鼠单抗
背景:    BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
免疫原:   A synthetic peptide from the internal region of BRAF which includes the mutation of G469E, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes G469E mutant, but not wild type BRAF of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant BRAF (G469E) and wild type proteins.Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRAF(G469E) monoclonal antibody (货号:   26196).Immunofluorescence:  Immunofluorescence of cells expressing BRAF proteins with Anti-BRAF(G469E) antibody.HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with Anti-BRAF(G469E) monoclonal antibody (货号:   26196).