| 货号: S219937 |
| 产品全名: YWHAQ 兔多抗 |
| 基因符号 1C5, HS1, 14-3-3 |
| UNIPROT ID: P27348 (Gene Accession – NP_006817 ) |
| 背景: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5′ UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. |
| 抗原: Synthetic peptide of human YWHAQ |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219937(YWHAQ Antibody) at a dilution of 1/20(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 219937(Anti-YWHAQ Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 219937(Anti-YWHAQ Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D260654(Anti-YWHAQ Antibody) at dilution 1/20. | 
Gel: 10+12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Mouse brain tissue, A549 cells, human lymphoma tissue, NIH/3T3 cells, human colon cancer tissue;
Primary antibody: 219937(YWHAQ Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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