武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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VIM Rabbit pAb

Anti-VIM Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216356

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216356
产品全名:   VIM 兔多抗
基因符号   
UNIPROT ID:   P08670 (Gene Accession – BC000163 )
背景:   This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
抗原:   Fusion protein of human VIM
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer, Neuroscience, Stem Cells
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 216356(VIM Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 216356(Anti-VIM Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate:40 μg;
Lane 1-2: 293T and Hela cell lysates;
Primary antibody: 216356(VIM Antibody) at dilution 1/300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second
           引用文献