武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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USP5 Rabbit pAb

Anti-USP5 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217993

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217993
产品全名:   USP5 兔多抗
基因符号   ISOT
UNIPROT ID:   P45974 (Gene Accession – BC004889 )
背景:   Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).
抗原:   Fusion protein of human USP5
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217993(USP5 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217993(Anti-USP5 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217993(Anti-USP5 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D223514(Anti-USP5 Antibody) at dilution 1/80.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: LNCAP cell lysate;
Primary antibody: 217993(USP5 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
           引用文献