| 货号: S219477 |
| 产品全名: UBE2O 兔多抗 |
| 基因符号 E2-230K |
| UNIPROT ID: Q9C0C9 (Gene Accession – BC022237 ) |
| 背景: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate ‘Lys-63’-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). |
| 抗原: Fusion protein of human UBE2O |
| 经过测试的应用: ELISA, IHC |
| 推荐稀释比: IHC: 100-300; ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219477(UBE2O Antibody) at a dilution of 1/120(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 219477(Anti-UBE2O Antibody) at dilution 1/120. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 219477(Anti-UBE2O Antibody) at a dilution of 1/120. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D227298(Anti-UBE2O Antibody) at dilution 1/120. |
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