| 货号: S218146 |
| 产品全名: TPPP 兔多抗 |
| 基因符号 p24; p25; TPPP1; TPPP/p25; p25alpha |
| UNIPROT ID: O94811 (Gene Accession – BC131506 ) |
| 背景: Tubulin family members are globular proteins important in the assembly of microtubules. Microtubules are structural components that play important roles in mitosis, cytokinesis and vesicle transport. TPPP (Tubulin polymerization-promoting protein), also known as p24 and p25, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm. TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, polymorphic aggregates, or stabilized blocks. TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope. TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. |
| 抗原: Full length fusion 蛋白 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218146(TPPP Antibody) at a dilution of 1/25(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218146(Anti-TPPP Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 218146(Anti-TPPP Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D223819(Anti-TPPP Antibody) at dilution 1/25. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal brain tissue ;
Primary antibody: 218146(TPPP Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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